Fig. 5

Western blot analysis for the detection of protein levels of ColA1, osteocalcin (OCN), and Sox-9 on 21 post-encapsulation days. The results revealed that the encapsulation of human MG-63 cells inside Alg-nHA-Col microspheres could alter osteogenesis-related factors such as ColA1 and osteocalcin. The β-actin-specific band is used as a standard. One-way ANOVA analysis and Turkey post hoc test (*p < 0.05; ** p < 0.01)