Fig. 3

Absorption and multiphoton fluorescence spectra of PSMA loaded with dyes with high molar masses. a UV–Vis spectra and b single-photon fluorescent spectra (excited at 512 nm) of 242, YI-1 (solid line), PSMA-242, and PSMA-YI-1 NPs (dotted line). c Fluorescence spectra of ADF1 and PSMA-ADF1 NPs excited at 512 nm. Multiphoton fluorescence spectra of d PSMA-242, e PSMA-YI-1, and f PSMA-ADF1 NPs