Fig.5
Various Ca2+ signals and PN production are involved synergistically in SUR1-TRPM4 activation and Na+ influx in response to AngII or BK stimuli. A, C Electrophysiological analysis of whole-cell currents in A549 cells. The voltage-ramp protocol for − 100 mV to 100 mV at a holding potential of 0 mV was provided at 2 s intervals. Representative current–voltage relationships during the application of AngII (10 μM) or BK (10 μM), with MCC950 (10 μM), and SAR7334 (10 μM). B, D The current densities averaged at potentials of + 80 Mv under AngII or BK, co-treatment of AngII or BK, with SAR7334 (10 μM), GSK2193874 (10 μM), Nifedipine (30 μM), Z-VAD-FMK (20 μM), JK (1 μM), and MCC950 (10 μM). E–G Changes in fluorescence intensity of ENG (Ft/F0) in cells under the different treatments. Average of ≥ 5 experiments ± SEM. ns, p > 0.05, **p < 0.01, ***p < 0.001