Fig.7

Effect of drug combinations on permeability and α-ENaC in alveolar epithelial and vascular endothelial cells stimulated with AngII or BK. A–D Normalized TEER values of each group; TEER value measured in the control group was defined as 100%. A549 and HPMEC cells were treated AngII (10 μM) or BK (10 μM), with SennosideA, Tranilast, SAR7334, and Gli for 90 min (A, C) and 24 h (B, D), respectively. E Quantification of EB exosmosis concentration (μM) in mouse lungs. F Protein concentration in bronchoalveolar lavage fluid was changed in the lungs of mice after treatment with AngII or BK and the drug combinations. G Summarized data for whole-cell current density elicited by a test potential at − 100 mV in the absence and presence of amiloride (10 µM). H ENaC-α subunit protein levels in cell lysates, as detected using western blotting. I Na, K-ATPase activity in A549 cells. Average of ≥ 5 experiments ± SEM. ns, p > 0.05, **p < 0.01, ***p < 0.001