Fig. 4

Inhibiting autophagy weakened the protective effect of huc-MSCs-derived exosomes on attenuating BV2 cells pyroptosis. a BV2 cells were administered LPS (1 µg/ml) for 1 h, 6 h, 12 h, 24 h and ATP (5 mM) for 30 min, and LC3, P62 were tested by western blot at the indicated time points. b Compared with con group, the protein levels of LC3-II and beclin1 were increased in 10 and 100 µg/ml exosomes pretreated BV2 cells, and the expression of P62 was inhibited. c, d Expression of NLRP3, GSDMD-F, GSDMD-N, caspase1-p20 was tested by western blot and subsequent quantitative analysis of the proteins as normalized to the LPS + ATP group. e BV2 cells pretreated with LPS, ATP, huc-MSCs-derived exosomes, 3-MA were stained with Yo-Pro-1 and Hoechst, bar = 50 µm. Data are presented as the mean ± standard deviation (SD). (*P < 0.05, **P < 0.01, ***P < 0.001)