Fig. 5

Cell proliferation and GFAP in astrocytes on MW-CNTs. A Cerebellar astrocyte cultured on PDL and CNT platforms 24 h after seeding were examined using confocal microscopy and immunofluorescence with DAPI (blue), anti-BrdU antibodies (green) and anti-GFAP antibodies (red). Scale bar, 50 μm. B The graph shows that astrocytes cultured on CNT platforms have more BrdU-positive DAPI spots than PDL (n = 45 cells). C The graph shows astrocytes 24 h after seeding on PDL and CNT platforms. There are more viable cells on CNT platforms than PDL. D The represented mRNA levels of astrocytes on CNT platforms were normalized to that of the control group and appeared as fold changes. In primary astrocytes on CNT platforms, gfap mRNA levels were significantly upregulated in 24 h than PDL (n = 4 from three independent cell preparations). E GFAP protein levels in cerebellar astrocytes significantly increased, as shown using western blotting analysis (n = 3 from three independent cell preparations)