Fig. 5

The nanotopography and the glycocalyx configuration affect the lamellipodial molecular clutch engagement and actin dynamics. A The graphs summarise the evolution of N_j, < F_j > and jump force distribution comparing untreated PC12 cells interacting with ns-Zr15 (red lines, reproduced from Fig. 4B,C), or treated the ROCK inhibitor Y27632 (10 µM, green lines or bars, for ns-Zr15 Native glycocalyx jump force distribution, compare with Fig. 3D). The error bars represent the effective standard deviation of the mean (details in 4.3.4). Asterisks indicate significant differences between control and Y27632 treatment. B The panel shows representative actin flowfield images obtained after Particle Image Velocimetry (PIV) of live cell recordings of PC12 cells (transfected previously with LifeAct™-mCherry to visualise the actin dynamics) in the different experimental conditions (corresponding videos can be found in the SI). The confocal recordings had a frame rate of 0.5 images/sec. The graph below shows the according PIV-based quantification of the actin dynamics. The boxplots show medians, 25th and 75th percentile as box limits. 198–234 frames from 11–14 cells/lamelllipodial zones were quantified. A Kruskal–Wallis statistical test was applied with multiple comparisons (flat-Zr native glycocalyx vs. after glycocalyx digestion, ns-Zr15 native glycocalyx vs. after glycocalyx digestion, flat-Zr vs. ns-Zr15 native glycocalyx, flat-Zr vs. ns-Zr15 after glycocalyx digestion). * p < 0.0001