Fig. 6

Laser irradiation induces robust therapeutic effects of Stealth Exo@Dox in vivo. A Schematic of experimental design. Balb/c mice were implanted with 5 × 10⁶ A20 cells subcutaneously. Exosomes were injected at the 24th day after tumor cell implantation. 532 nm laser irradiation (0.5 W cm⁻², 10 min) was performed on the tumor region right after exosome injection and at the 4 h and 12 h post-injection. B–D Representative images of histological analysis of the tumor tissues in mice with indicated treatments. Mice were sacrificed after twice treatments. H&E (B), DCFH (C), and TUNEL (D) were performed. Scale bar = 200 μm. n = 6 for each group. E qPCR analysis of ferroptosis related genes in tumor tissues in different groups. Gapdh served as internal controls. Data shown are representative of 6 mice and expressed as mean ± SEM. *p < 0.05 by one-way ANOVA. F Representative images of excised tumors in mice treated as indicated. Mice were sacrificed at the end of the experiment. n = 10. G Tumor volume of tumors on Balb/c mice post various treatments (n = 10). H The survival curve of tumor-bearing Balb/c mice with different treatments. (n = 10 mice/group). *p < 0.05