Fig. 4
From: Remodeling of tumor microenvironment for enhanced tumor chemodynamic/photothermal/chemo-therapy
a Temperature variation curve of different concentrations of AFZD NPs (0.25 ~ 1.00 mg/mL) under the irradiation of a NIR laser (808 nm, 1.5 W/cm2) for 600 s. b Temperature elevation of the aqueous AFZD with different concentrations (0, 0.25, 0.5, 0.75, 1.0 mg/mL) under the irradiation of a NIR laser (808 nm, 1.5 W/cm2) for 600 s and shutting off the laser. c The determination of the time constant for heat transfer from the system using linear regression of the cooling profile of the AFZD with 0.5 mg/mL concentration. d Cyclic photothermal stability of AFZ NPs. e Laser scanning confocal microscopy images of HCT116 cells cultivated with AFZ NPs. Scale bar = 50 μm. h Infrared images of mice after administration of PBS buffer and AFZ NPs and the temperature changes with irradiation time (808 nm, 1.5 W/cm2). f Viability of HCT116 cells treated with the same DOX concentrations in AFZD NPs and free DOX (n = 4, mean ± s.d. p < 0.05). g Viability of HCT116 cells treated with different concentrations of AFZ, AFZD and AFZDA NPs (n = 4, mean ± s.d. p < 0.05) under different conditions. i Cumulative DOX release from AFZD NPs (0.75 mg/mL) at different pH values (n = 4, mean ± s.d.)