Fig. 3

CS-PLGA NP-mediated anti-inflammatory and cellular uptake in vitro. Cytotoxicity of the NPs in Caco-2 cells (A) and RAW264.7 cells (B). Histogram (C) and mean fluorescence intensity (D) of the NP-internalized Caco-2 cells were analyzed by flow cytometry. E Fluorescence images of Caco-2 cells after incubation with the coumarin 6-labeled NPs (scale bar: 50 µm). F ROS levels were reduced in the LPS-induced RAW264.7 cells after drug treatment. G IFN-γ levels decreased in drug-treated M1Φ