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Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: Directing ricin-based immunotoxins with targeting affibodies and KDEL signal peptide to cancer cells effectively induces apoptosis and tumor suppression

Fig. 5

Construction of AlBD-SC-RTA-KDEL by genetic fusion of AlBD and SC, and subsequent target ligand addition via an SC/ST protein ligation system. A Schematic illustration and B SDS-PAGE analysis of post-translationally ligated resultants between AlBD-SC-RTA (50.0 kDa) and ST-HER2Afb (10.3 kDa) or ST-EGFRAfb (10.1 kDa), or between AlBD-SC-RTA-KDEL (50.4 kDa) and ST-HER2Afb or ST-EGFRAfb, forming AlBD/HER2Afb/RTA (60.3 kDa), AlBD/EGFRAfb/RTA (60.1 kDa), AlBD/HER2Afb/RTA-KDEL (60.7 kDa) and AlBD/EGFRAfb/RTA-KDEL (60.5 kDa), respectively. In vitro measurements of dose-dependent cytotoxicity of AlBD-containing RITs in C SK-BR-3 and D NIH3T6.7 cells, using CCK-8 assay. Each construct was indicated

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Journal of Nanobiotechnology

ISSN: 1477-3155

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