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Fig. 6 | Journal of Nanobiotechnology

Fig. 6

From: Vancomycin-conjugated polydopamine-coated magnetic nanoparticles for molecular diagnostics of Gram-positive bacteria in whole blood

Fig. 6

Improvement in the sensitivity of qPCR (a) and PCR with gel electrophoresis (b–d) to detect S. aureus in spiked blood samples through the preconcentration of the microorganism using van-PDA-MNPs. Ct value plotted against S. aureus at different concentrations (101–104 CFU/mL) in spiked blood samples with and without the preconcentration by van-SiO2-MNPs or van-PDA-MNPs. b–d Gel electrophoresis images of the amplified DNA (207 bp) of the target gene (nuc) by PCR with (c, d) and without (b) the preconcentration

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