Fig. 7
(Reprinted with permission from Ref [86] Copyright © 2020, Journal of Nanobiotechnology, Jun Wang et al.)
Higher level of exosomal PD-L1 in OS patients, especially those with pulmonary metastasis, compared to healthy donors and patients with benign tumor (BT). a A TEM image of exosomes immunogold-labelled with PD-L1 antibody. b Western blot analysis of PD-L1 expression in exosomes. c The levels of PD-L1 in serum exosomes from healthy donors and patients with OS and BT by ELISA. d ROC analysis showed that the AUC of serum-derived exosomal PD-L1 was 0.695, which could distinguish OS patients from healthy controls. e, f Higher expression of exosomal PD-L1 and N-cadherin in OS patients with metastasis than those without metastasis via western blot and ELISA analysis. g Radiological images of 4 OS patients with and without pulmonary metastasis