Fig. 8
(Reprinted with permission from Ref [95] Copyright © 2022, Frontiers in oncology, Xin Hu et al.)
Exosomal lncRNA ANCR dictates in vitro sensitivity of sarcoma to doxycycline, expression of which is critical for drug resistance, tumor progression, and overall survival of patients. A Relative growth rate of the drug resistant cells mock-transfected, or transfected with non-silencing control siRNA, or siRNA targeting DANCR were quantified using CCK8 assay. b Cell viability of the differently transfected drug resistant cells after treated with doxycycline. c Representative IHC staining of osteocalcin. Nude mice were injected with sarcoma cells pre-treated with exosomes and added to sarcoma cells. All mice were treated with doxycycline orally once every 3 days for 4 weeks. Knockdown of DANCR made sarcoma cells sensitive to doxycycline and inhibited lung metastasis. d The tumor growth rate. e Expression of DANCR in OS patients sensitive or resistant to Adriamycin (n = 10 each) via using RT-qPCR. f Kaplan–Meyer survival curve showed significantly higher overall survival in patients with lower ANCR expression (median survival 4.56 years) than those with higher ANCR expression (median survival 2.26 years; P = 0.0071)