Fig. 2

hsEVs attenuated ADSC cell death caused by H₂O₂ stress. A A CCK8 assay was conducted to detect the viability of ADSCs in the presence of different concentrations of nsEVs and hsEVs under exposure to 300 µM of H₂O₂ for 12 h. B A live/dead assay was performed to detect cell death of ADSCs under ROS pressure after preconditioned by PBS, nsEVs (20 µg/mL) and hsEVs (20 µg/mL). C Quantitative analysis of the live/dead assay performed in B. D The protein level was detected in ADSCs, ADSCs under H₂O₂-induced stress, ADSCs preconditioned by nsEVs or hsEVs for 12 h and then exposed to H₂O₂ (300 nM). E The expression of PTEN was detected by western blotting. F The apoptotic rate of ADSCs was measured by FCM. All data are representative of three independent experiments and are shown as the mean ± SEM. (n = 3; *P < 0.05; **P < 0.01).