Fig. 1

Biogenesis and identification of small extracellular vesicles (sEVs). sEVs originate from the endosomal pathway by the formation of endosomes and MVBs. When MVBs fuse with cell membrane, sEVs are released into extracellular milieu. sEVs are composed of a lipid bilayer vesicle containing nucleic acids, proteins, lipids, glycans, and other small molecules