Fig. 6
From: BMSC exosome-enriched acellular fish scale scaffolds promote bone regeneration
Enhanced osteogenesis of DE-FS with OBMSC-Exos encapsulation in vitro. (a) The exosome loading efficiency of DE-FS as determined by detecting the concentration of exosomes in the medium. (b) Exosomes released from DE-FS loaded with exosomes were determined by detecting the concentration of exosomes in the basal medium. (c) SEM images of DE-FS cultured with OBMSC-Exos for 24 h. Scale bar was 20 μm (left panel) and 2 μm (right panel). (d) Confocal images of DE-FS cultured with PKH26-stained OBMSC-Exos. Scale bar was 20 μm. (e) Confocal images of BMSCs cocultured with DE-FS treated as in Fig. 5D. (f) Q-PCR analysis of Runx2, Col1a1 and OPN expression in BMSCs cultured on the surface of DE-FS with OBMSC-Exos encapsulation. (g) Western blot analysis of RUNX2, Collagen I and OPN expression in BMSCs cultured on the surface of DE-FS with OBMSC-Exos encapsulation. (h) Alizarin red staining of BMSCs cultured on the surface of DE-FS with or without OBMSC-Exos encapsulation. Scale bar was 200 μm