Fig. 3

MiR-140-5p inhibits osteogenic differentiation of hMSCs. a Photos of GFP-positive OPLL cells under fluorescence microscopy indicated that OPLL cells were successfully transinfected with lentivirus. Scale bars = 10 μm. b Relative expression of miR-140-5p in exosomes derived from OPLL cells transfected with LV-miR-140-5p, LV-miR-NC, LV-sponge, or LV-sponge-NC. c Relative expression of miR-140-5p in hMSCs, which was delivered by exosomes derived from OPLL cells. The inhibitory effect of miR-140-5p on osteogenesis was confirmed by ALP staining d and Alizarin red staining e. The relative expression of osteogenesis-related genes was assessed by qPCR f and Western blotting g All experiments were repeated three times. GAPDH served as the internal control. *P < 0.05. All data are presented as the mean ± SD. LV, lentivirus. NC, negative control. GFP, green fluorescent protein