Fig. 4

Testing of Serpin-loaded EV activity in vitro. a Fusions of an amino terminal myristoylation sequence with each Serpin were cloned into lentiviral vectors. b-d Validation of three Serpin-loaded EVs by immunoblotting in cultured media. Sham is PBS and Empty is the empty vector used for the myristoylation fusions. e Representative images of HaCaT cell closure kinetics. f Gap closure quantification of HaCaT cells treated with SERPINA1-EVs, SERPINF2-EVs, SERPING1-EVs, vs. empty vector, and sham (PBS). (p-value: *** < 0.001, n = 6). Statistical analysis of scratch assay on HaCaT cells by SERPIN-loaded EVs enriched from HEK293 donor cells on g 2 h, h 4 h, i 24 h (p-value: ** < 0.005, * < 0.05)