Fig. 4
miR-CM1 in FELNVs exert anti-aging effects. a Comparison of expression levels of the five novel miRNAs of FELNVs with/without oxidation treatment. b Effect of five novel miRNAs on proliferation in UV-treated HaCaT cells by CCK8 assay. c Relative SA-β-Gal levels in UV-treated HaCaT cells with miR-CM1 or miR-CM3. d Effect of miR-CM1 on mRNA expression levels of MMP1 and COL1A2 in UV-treated HaCaT cells was tested by qRT-PCR. e Effect of miR-CM1 on the expression of MMP1 and COL1A2 proteins in UV-treated HaCaT cells was tested by Western blot. f–h Relative ROS levels (f), MDA content (g) and relative SOD enzyme activity (h) in UV-treated HaCaT cells with miR-CM1 treatment. i Flow diagram to show 3D cell printing. j Representative imaging of H&E stain of skin tissue from 3D printing and cultures. Data are expressed as mean ± SD (*P < 0.05, **P < 0.01)