Fig. 6

miR-CM1 resists UV-induced aging by inhibiting Mical2 expression. a mRNA expression levels of Mical2 in HaCaT cells with miR-CM1 transfection tested by qRT-PCR. b Protein expression of Mical2 in HaCaT cells with miR-NC or miR-CM1 transfection were tested by Western blot. c Protein expression of Mical2 in HaCaT cells with Mical2 transfection or co-transfection of Mical2 and miR-CM1 were tested by Western blot. d ROS levels in HaCaT cells with Mical2 transfection and co-transfection of Mical2 and miR-CM1. e Representative imaging of IF in HaCaT cells labeled for MMP1 (green), COL1A2 (red), and nuclear (DAPI: blue) in different treatment groups. f mRNA expression levels of MMP1 and COL1A2 in HaCaT cells with miR-CM1 transfection and co-transfection of Mical2 and miR-CM1 tested by qRT-PCR. g, h SOD enzyme activity (g) and relative SA-β-Gal levels (h) in HaCaT cells with miR-CM1 transfection and co-transfection of Mical2 and miR-CM1. Data are expressed as mean ± SD (*P < 0.05, **P < 0.01)