Fig. 8

Evaluation of whole-mount immunostaining of the crushed nerve on post-injury day 21 for nerve fiber, myelin, and blood vessel distribution. A Representative compact images of immunofluorescence staining of the whole nerve for MPZ in orange, NF-H in green, CD31 in red, merged view, and region of interest (ROI) in the injury zone. B For the purpose of evaluation and quantification, each imaged whole nerve was divided into 3 zones: proximal, injury, and distal. The white box represents the injury ROI (n = 5/group; magnification = 5x; scale bar = 500 μm). Each image represents 3 images from 5 different SNs for a total of 15 images per group