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Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: Cytoplasmic delivery of siRNA using human-derived membrane penetration-enhancing peptide

Fig. 5

Intracellular delivery of Ago2 fusion proteins. a DNA constructs for Ago2 fusion proteins. b Confocal images of HeLa cells treated with 100 nM of Cy3-labelled Ago2 fusion proteins (red) for 1 h. Nuclei were stained with Hoechst 33,342 (blue). Scale bars, 20 μm. c ROI was taken for each cell and the average fluorescence intensity of Cy3 was quantified. N = 40 or 54; means ± SD; **p < 0.01

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