Fig. 2

Cell internalization of PSSP@ART-ISMN and its cytotoxicity in vitro. A CLSM images of SKOV3 cells treated with PSSP@Rh B for 2, 4, and 6 h, scale bar = 25 µm. Flow cytometry (B) and semi-quantitative analysis (C) of SKOV3 cells after incubation with PSSP@Rh B for 2, 4, and 6 h. D Relative cell viability of SKOV3, HO8910, and IOSE-80 cells after treatment with ART, ART-ISMN, PSSP@ART, and PSSP@ART-ISMN for 72 h. Statistical analyses were carried out between free ART group and PSSP@ART-ISMN NPs group. E SKOV3 cell apoptosis images after different treatments for 24 h. *P < 0.05, **P < 0.01, and ***P < 0.001; ns indicates P > 0.05