Fig. 4

NanoS100-SwIAV altered the frequencies of T-lymphocytes in TBLN MNCs and BAL cells of vaccinated pigs. Five-week-old SPF pigs were administered twice ID/IM with NanoS100-SwIAV, Nano11-SwIAV or control NanoS100 and challenged at post-prime vaccination day 35 with SwIAV H1N1-OH7. TBLN MNCs and BAL cells were isolated at DPC6 and were restimulated with SwIAV H1N1-OH7 in vitro. The cells were labeled and analyzed by flow cytometry for the frequencies of T-lymphocyte subsets: A Total IL-17A⁺ CTLs-IM [CD3⁺CD4⁻CD8α⁺CD8β⁺IL-17A⁺]; B IL-17A⁺ Late effector CTLs-IM [CD3⁺CD4⁻CD8α⁺CD8β⁺CD27⁻IL-17A⁺]; C Total IFNγ⁺ CTLs-IM [CD3⁺CD4⁻CD8α⁺CD8β⁺IFNγ⁺]; D IFNγ⁺ Early effector CTLs-IM [CD3⁺CD4⁻CD8α⁺CD8β⁺CD27⁺IFNγ⁺]; E IFNγ⁺ Late effector CTLs-IM [CD3⁺CD4⁻CD8α⁺CD8β⁺CD27⁻IFNγ⁺] in TBLN MNCs; F Total CTLs-ID [CD3⁺CD4⁻CD8α⁺CD8β⁺]; G Early effector CTLs-ID [CD3⁺CD4⁻CD8α⁺CD8β⁺CD27⁺]; H Central memory T-helper/memory cells-IM [CD3⁺CD4⁺CD8α⁺CD8β⁻CD27⁺] in BAL cells. Data represent the mean value of 7–8 pigs ± SEM. Statistical analysis was performed by one-way ANOVA followed by Tukey’s post-test. For head-to-head comparison between two groups, Unpaired t-test was used. Asterisk/Pound represents significant difference between indicated groups (*/♯ p < 0.05, **/♯♯ p< 0.01, and ***/♯♯♯ p < 0.001)