Fig. 7

CDs inhibited NF-κB and MAPK pathway activation in OA chondrocytes. A Western blot assay indicated CDs inhibited NF-κB pathway activation in OA chondrocytes. B, C Immunofluorescence assay showed CDs inhibited the nuclear translocation of p65 in chondrocytes with IL-1β stimulation. Scar bar: 20 μm. D Western blot assay indicated CDs administration could partially suppress the translocation of p65 from cytoplasm to nucleus caused by IL-1β stimulation. E The relative band intensity of p65 in the nucleus relative to the cytoplasm based on Image J software. F Western blot assay results indicated CDs inhibited MAPK pathway activation in OA chondrocytes. G The relative band intensity of proteins involved in MAPK pathway was calculated by Image J software. *p < 0.05; **p < 0.01; ***p < 0.001