Fig. 6

PTPN maintained mitochondrial respiratory chain function. a Mitochondrial membrane potential (JC-1) of primary cardiomyocytes treated with PTPN. Red indicated JC-1 aggregates and green indicated JC-1 monomer. (n = 3, scale bar = 20 μm). b Quantitative results of mitochondrial membrane potential. c Western blot analysis of OxPhos complexes in primary cardiomyocytes treated with PTPN. Relative protein expression of OxPhos subunits: NDUFB8 (d), SDHB (e), UQCRC2 (f), MTCO1 (g) and ATP5A (h) (n = 3). i ATP level of primary cardiomyocytes treated with PTPN (n = 4). Data are presented as mean ± SEM. Statistical analysis was via one-way ANOVA with GraphPad Prism 8.0; ns: not significant; *p < 0.05; **p < 0.01; and ***p < 0.001