Fig. 3

Cellular uptake mechanism and intracellular colocalization of Apt-NS. A Confocal images showing the influence on Apt-NS uptake in A549 cells treated by different endocytic inhibitors. B Quantitative analysis of the mean fluorescence intensity of each group in (A) (n = 3). Statistical analysis: ***P < 0.001. C Flow cytometric assay showing the influence on Apt-NS uptake in A549 cells treated by different endocytic inhibitors. D Quantitative analysis of the mean fluorescence intensity of each group in (C) (n = 3). Statistical analysis: ***P < 0.001. E Confocal images showing colocalization of Apt-NS with lysosome. F The fluorescence intensity of LysoTracker (green) and Cy5-labeled Apt-NS (red) at the same site of marker positions (white line) in the overlay image of (E). Scale bar is 25 μm