Fig. 8

Anti-metastasis ability assay of CPCCM NPs. A Tumor growth curves of 4T1 tumor-bearing mice with different treatment. B Fluorescence images of 4T1 tumors. C The average tumor weight of different groups (n = 5). D Variation of body weight of tumor-bearing mice with different treatments. E Photographs of liver and lung tissues. F H&E staining of the lung with different treatments (the red arrow indicated metastatic nodules). G H&E stained the liver with different treatments (the red arrow indicated metastatic nodules). H Immuno-histochemistry staining of MMP-9 and HIF-1α in tumor tissues. Data were shown as means ± SD, n = 5. ANOVA was used to assess statistical significance. *p < 0.05, **p < 0.01, ***p < 0.001