Fig. 4

Neutralization of merozoites. A Purification of mature iRBCs with Percoll separation solution, illustrating iRBCs (red arrow) and normal erythrocytes (blue arrow). B, C Uptake of Lip-ARM, EM-ARM, and PEM-ARM by merozoites in pbANKA-infected mice (n = 3, ^* P < 0.05 and ^** P < 0.01). D Confocal fluorescent images of merozoites (blue) and PEM-ARM (red); scale bar = 20 μm (general), scale bar = 2 μm (magnification). E Representative scatter plots of Hoechst 33,342/CFDA-SE analysis of the cytoadherence assay of merozoites and normal erythrocytes after drug treatment. F Percentage of Q₂ area cells (n = 3, ^* P < 0.05 and ^** P < 0.01)