Fig. 4

Influence of MG63 cytoskeleton on gene transfection in microcircles and non-patterned surface. a Illustration of gene transfection by incorporating plasmid with liposome into MG63 cells for intercellular delivery, nuclear transcription and GFP protein translation. b Representative fluorescence images of transfected MG63 cells expressing GFP proteins (green). Nucleus: blue; actin bundles: red. Scale bar: 50 μm. c Gene transfection efficiency by analyzing the amount of GFP-positive cells. The data present the mean ± SD, n = 5, N.S. present no significance, *p < 0.05, ***p < 0.001