Fig. 3

Increased-infiltration of Treg cell in spinal cord promotes functional recovery and limits microglia pyroptosis after SCI. A BMS scores during 28 days of recovery after SCI demonstrate better functional recovery in SCI + Treg mice (n = 6); B Footprint analysis at Day 28 postinjury demonstrate better functional recovery in SCI + Treg mice(n = 6); C The footprints quantifcation of mice walking 28 days after SCI; D Rotarod testsat Day 28 postinjury demonstrate better functional recovery in SCI + Treg mice(n = 12); E MEP analysis was used as electrophysiological assessment in SCI + PBS and SCI + Treg mice at Day 28 postinjury (n = 6); F Quantification of peak-to-peak MEP amplitudes and latencies in SCI + PBS and SCI + Treg mice. G Representative immunofluorescence images for GSDMD (pyroptosis) and IBA1 (microglia) expression in SCI + PBS and SCI + Treg mice at Day 7 postinjury. H Representative immunoblots showing levels of pyroptosis-related protein in injured spinal cord at days 7 postinjury in SCI + PBS and SCI + Treg mice