Fig. 8

Exosomal miR-709 supress microglia pyroptosis by regulating NKAP expression. A Exosomal miR-709 regulates NKAP by directly targeting the 3′-UTR; B Luciferase report assay was performed to confirm NKAP is the target gene of miR-709; C The mRNA level of NKAP in BV2 cells after treatment with miR-709^OE-Exos and miR-709^KD-Exos; D The protein level of NKAP in BV2cells after treatment with miR-709^OE-Exos and miR-709^KD-Exos; E–G Rescue experiments for miR-709 inhibition were conducted by downregulating NKAP in microglia. microglia pyroptosis was detected by immunofuorescence (E, F) and western blot analysis (G); H–J Rescue experiments for miR-709 overexpression were carried out by the ectopic expression of NKAP in microglia. microglia pyroptosis was detected by immunofuorescence (H, I) and western blot analysis (J)