Fig. 4

Attenuation of inflammation by IFN-γ-iMSC-EVs in AD mice. AD mice were subcutaneously administered with PBS or IFN-γ-iMSC-EVs (50 or 500 μg) for negative control and test groups, respectively. A Distribution and number of mast cells in skin tissues. Mast cells in the skin layer were examined by staining with Toluidine blue. Scale bar: 100 μm. n = 5. Data are presented as mean ± SE. ***p < 0.001; ^###p < 0.001. B Analysis of inflammatory cell number in the skin layer of AD mice that received PBS or IFN-γ-iMSC-EVs. n = 5. Data are presented as mean ± SE. ***p < 0.001; ^###p < 0.001. C Immunoblot analysis of TSLP in the skin tissues collected from AD mice that received PBS or IFN-γ-iMSC-EVs. n = 5. Data are presented as mean ± SE. **p < 0.01; ^###p < 0.001. D Immunoblot analysis of CD23 and FcεRI in skin tissues of AD mice that received PBS or IFN-γ-iMSC-EVs. n = 5. Data are presented as mean ± SE. *p < 0.05; ^##p < 0.01; ^###p < 0.001. E Immunoblot analysis of phosphorylated p65 in skin tissues from AD mice that received PBS or IFN-γ-iMSC-EVs. The density of phosphorylated p65 was normalized to that of total p65. n = 5. Data are presented as mean ± SE. ***p < 0.001; ^##p < 0.01