Fig. 6

Restoration of skin barrier and lipid synthesis by IFN-γ-iMSC-EVs in the epidermis of AD mice. AD mice were subcutaneously administered with PBS or IFN-γ-iMSC-EVs (50 or 500 μg) for negative control and test groups, respectively. A Microscopic images of the skin tissues collected from IFN-γ-iMSC-EVs-injected AD mice. Tissues were stained with hematoxylin and eosin. The thickness of the epithelium was compared. Scale bar: 100 μm. n = 5. Data are presented as mean ± SE. ***p < 0.001; ^##p < 0.01. B Immunoblot analysis of skin barrier-related proteins in the epidermis of AD mice that received PBS or IFN-γ-iMSC-EVs. n = 5. Data are presented as mean ± SE. **p < 0.01; ^#p < 0.05; ^##p < 0.01. C Immunoblot analysis of proteins involved in lipid synthesis in the epidermal tissue of AD mice that received PBS or IFN-γ-iMSC-EVs. n = 5. Data are presented as mean ± SE. ^##p < 0.01; ^###p < 0.001