Fig. 2
From: Acidic and hypoxic tumor microenvironment regulation by CaO2-loaded polydopamine nanoparticles
In vitro treatment against 4T1 cells. A Relative cell activity after dealing with raised concentration of CaO2@mPDA-SH or mPDA nanoparticles. B Time-dependent confocal photos of 4T1 cells treated with CaO2@mPDA-SH (100 µg mL−1) or mPDA (100 μg mL−1) nanoparticles. Scale bar: 100 μm. C Time-dependent cellular uptake of 4T1 cells treated with CaO2@mPDA-SH (100 μg mL−1) nanoparticles examined by flow cytometry. D Apoptosis ratios of flow cytometry apoptosis experiment based on annexin V-FITC/PI staining of 4T1 cells after incubation with different concentration of CaO2@mPDA-SH or mPDA nanoparticles. E CLSM images of 4T1 cells with hypoxia detection probes [Ru(dpp)3]Cl2 in different treatments. Scale bar: 50 μm. F Quantitative analysis of mean fluorescent intensity of treated cells of E (n = 5, mean ± SD). G Lactic acid consumption effect of mPDA, Ca(OH)2@mPDA-SH, CaO2@mPDA, and CaO2@mPDA-SH nanoparticles at cellular level