Fig. 4

Immune analyses. A Representative FACS plots of tumor infiltration T cells (gated by CD3). B Representative FACS plots of tumor infiltration M1 macrophages (gated by CD11b). C Representative FACS plots of mature DCs in tumor-draining lymph nodes (gated by CD11c). D Representative FACS plots of T lymphocytes in the spleen (gated by CD3). E The ratio between CD3⁺CD8⁺ and CD3⁺CD4⁺ T cells in tumor for different groups according to the FACS data (n = 5). F The ratio between M1 and M2 macrophages of different groups according to the FACS data (n = 5). G Quantitative analyses of the CD80⁺CD86⁺ T cells in tumor-draining lymph nodes (n = 5). H The ratio between CD3⁺CD8⁺ and CD3⁺CD4⁺ T cells in spleen for different groups according to the FACS data (n = 5). In E–H, statistical significance was calculated through the one-way ANOVA with Tukey post-hoc analysis, *p < 0.05, **p < 0.01, ***p < 0.001