Fig. 2

Preparation and characterization of R4F-NM@F127. a Average size of NM@F127 and R4F-NM@F127. b Zeta potential of NM@F127 and R4F-NM@F127. c Stability of NM@F127 and R4F-NM@F127 monitored by DLS. DLS: dynamic light scattering. d Representative image of R4F-NM@F127 by TEM. An enlarged image was shown in the upper right. TEM: transmission electron microscopy. Scale bar, 100 nm. e LFA-1, L-selectin and CXCR2 on the neutrophil surface were detected by confocal imaging. Scale bar, 5 μm; Blue, DAPI; Red, LFA-1; Magenta, L-selectin; Green, CXCR2. f Characteristic protein bands of NMs and R4F-NM@F127 analyzed by western blotting. g White light images and UV-Vis absorption spectra of R4F-NM@F127, free DiR-BOA and DiR-BOA-labeled R4F-NM@F127. 1: R4F-NM@F127; 2: free DiR-BOA; 3: DiR-BOA-labeled R4F-NM@F127. h A series of images of DiR-BOA-labeled R4F-NM@F127 fluorescence at different concentrations. Data are presented as the mean ± SEM (n = 3)