Fig. 3

The SR-B1 receptor targeting ability of R4F-NM@F127. a Confocal imaging of the SR-B1 targeting ability of R4F-NM@F127 (20 µM). b Quantitative analysis of the MFI of DiR-BOA in ldlA7 cells and ldlA (mSR-B1) cells after incubation with DiR-BOA-labeled NM@F127 and R4F-NM@F127. c Confocal imaging of the ability of DiR-BOA-labeled R4F-NM@F127 to target RAW264.7 cells in vitro (20 µM). d Quantitative analysis of the MFI of DiR-BOA in RAW264.7 cells after incubation with DiR-BOA-labeled NM@F127 and R4F-NM@F127 at different concentrations (n = 3 for each group). e Confocal imaging of the ability of DiR-BOA-labeled R4F-NM@F127 to target BMDMs in vitro (20 µM). f Quantitative analysis of the MFI of DiR-BOA in BMDMs after incubation with DiR-BOA-labeled NM@F127 and R4F-NM@F127 at different concentrations (n = 3 for each group). Scale bar, 10 μm; Blue, DAPI; Green, phalloidin; Red, DiR-BOA; BMDMs, Bone marrow-derived macrophages; MFI: mean fluorescence intensity; Incubation time: 3 h. Data are shown as the mean ± SEM. **p < 0.01, and ***p < 0.001, as analyzed by unpaired t test