Fig. 2

Formation of primary tumor-derived organotypic cell clusters (POCCs) derived from primary mouse tumors. a Schematics show the comparison of POCCs and conventional organoids from mouse orthotopic breast tumors, respectively. b Formation of POCCs (day 1) and Matrigel organoids (day 28) with similar size. White scale bar: 500 μm; Black scale bar: 100 μm. c Growth of POCCs and Matrigel organoids from day 1 to day 4 and day 1 to day 28, respectively. d Size distribution in POCCs on day 1 and Matrigel organoids on day 28. e Cell components in POCCs, Matrigel organoids, and primary tumor tissues. DAPI (nucleus), ER (tumor), SMA (CAF), CD3 (immunocyte). Scale bar: 100 μm