Fig. 4

Evaluation of cancer immunotherapy using patient breast tumor tissues. a Coculture process of TILs and the dissociated tumor cells. b The typical killing results (left: control group; right: PEM-treated group) of T cells over 12 h along POCCs. The red color represents the dead cell, green color represents the T cell. c Statistical analysis of TIL’s killing efficiency in two groups among three breast cancer patients. n = 3 biological replicates, statistical analysis was performed using two-sample Student’s t-test, statistical significance was denoted as: ns p > 0.05, **p < 0.01, ***p < 0.005. Data are mean ± s.d. Scale bar: 200 μm