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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Magnetofection of miR-21 promoted by electromagnetic field and iron oxide nanoparticles via the p38 MAPK pathway contributes to osteogenesis and angiogenesis for intervertebral fusion

Fig. 3

Magnetofection of miR-21 promoted osteogenesis. A, B Alizarin red S (ARS) staining pictures 14 days after different treatments and the corresponding semi-quantification analysis. C Quantification analysis of alkaline phosphatase (ALP) activity 7 days after treatment. D–G Western Blotting analysis of Col 1, OCN, and OPN as well as corresponding semi-quantifications of protein expression levels of Col 1 (E), OCN (F) and OPN (G). H–J Relative mRNA expression levels of Col 1 (H), OCN (I), and OPN (J). K, L Immunofluorescence images of OCN (K) and Runx2 (L) after various treatments (PBS, lipofectamine 2000 (lipo 2000), electromagnetic field (EMF), PEI@INPs, EMF + PEI@IONPs). OCN/Runx2 was labelled with Cy3 (red), and nuclei were labelled with 4′,6-diamidino-2-phenylindole (DAPI, blue). *p < 0.05, **p < 0.01, ***p < 0.001 compared to the PBS group; #p < 0.05, ##p < 0.01 compared to the lipo 2000 group

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Journal of Nanobiotechnology

ISSN: 1477-3155

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