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Fig. 4 | Journal of Nanobiotechnology

Fig. 4

From: Magnetofection of miR-21 promoted by electromagnetic field and iron oxide nanoparticles via the p38 MAPK pathway contributes to osteogenesis and angiogenesis for intervertebral fusion

Fig. 4

Magnetofection of miR-21 promoted angiogenesis. A Assessment of the migratory activity of human umbilical endothelial cells (HUVECs) at 24 h by scratch wound assay; the red dashed lines are the edges of the cell migration. B Assessment of the cell migration rate of HUVECs by transwell assay. C Tube formation assay of HUVECs. D–G Quantitative analysis of wound size recovery rate (D), migration rate (E), total tube length (F) and branch points (G). *p < 0.05, **p < 0.01, ***p < 0.001 was compared to the PBS group; #p < 0.05, ##p < 0.01 was compared to the lipo 2000 group. H, I Immunofluorescence images of CD31 (H) and vWF (I) after various treatments. CD31 and vWF was labelled with Cy3 (red), and nucleus were labelled with DAPI (blue)

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