Fig. 4

Developmental toxicity evaluation of Nano-Se, Nano-S, Nano-Se@S in zebrafish. A Representative images of developmental toxicity in zebrafish treated with different concentrations Nano-Se; B Representative images of developmental toxicity in zebrafish treated with different concentrations of Nano-S; C Representative images of developmental toxicity in zebrafish treated with different concentrations of Nano-Se@S; Toxicity of Nano-Se D, Nano-S H, Nano-Se@S L was evaluated by survival rate; Toxicity of Nano-Se E, Nano-S I, Nano-Se@S M was evaluated by hatching rate; Toxicity of Nano-Se F, Nano-S J, Nano-Se@S N was evaluated by heart beats/1 min in 72 h; Toxicity of Nano-Se G, Nano-S K, Nano-Se@S O was evaluated by a body length in 72 h. (n = 10, Mean values ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)