Fig. 7

Angiogenesis promoted by Nano-Se@S. The 24 hpf embryos were treated with different concentrations of Nano-Se-S for 48 h. A Representative fluorescence microscopy images in transgenesis zebrafish (fli1:EGFP) showed blood vessels labeled with green fluorescence. B The quantitative average number of sprouting vessels treated with Nano-Se@S in transgenic zebrafish (fli1:EGFP) (n = 8). C Nano-Se@S promotes angiogenesis of the allantoic membrane of chicken embryos. D Quantitative vessel percentage area treated with Nano-Se@S in the chicken embryo (n = 5). E CD31 immunohistochemistry (IHC) shows vascularization within the Nano-Se@S granulation tissue. Arrow indicate angiogenesis (n = 5) (Mean values ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)