Fig. 2

BBB-oC devices after cell seeding with tri-culture. A Schematic representation of neurovascular cells arrangement into the BBB-oC B Optical images at Day 7 of ECs at 10 × and C Hydrogel zone at 20 × D Confocal z-projections of F-actin from cytoskeleton of neurovascular cells E Cell viability assay of ECs every day from day 3 of cell seeding at 20 × and hydrogel zone at Day 7 at 10 × F Optical images and G cell viability assay of endothelial cells at Day 7 into TEER-BBB-oC after TEER measurement every day at 10x. Scale bar 100 μm excepting E scale bar 50 μm F % Alive cell in the endothelial barrier over days and hydrogel zone at day 7