Fig. 3

PFR upregulates MHC-I in 4T1 cells. A Schematic diagram of MHC-I upregulation by ropivacaine. AP: autophagosome, AL: autolysosome, Rop: ropivacaine. B Fluorescent images of LC3 and lysosomes. Ad-GFP-LC3B-infected cells were treated with PF or PFR for 8 h and stained with Lyso-Tracker red (scale bar represents 5 μm). C Statistical results of the size of lysosomes from (B) (n = 4). D–F Western blot and statistical results of P62 and LC3II in 4T1 cells treated with PF or PFR for 8 h. G Flow cytometry results of the mean fluorescence intensity (MFI) of MHC-I on the 4T1 cell surface. H Statistical results from (G). I Immunofluorescent staining of MHC-I (red) and DAPI (blue) (scale bar represents 5 μm). PF PF127 hydrogel, PFR PF hydrogel mixed with ropivacaine