Fig. 5

PLGA NPs are phagocytized by ECs in time-dependent manner, decreasing cell viability and cell migration. A HUVECs uptake of PLGA NPs on 0.5, 2, 4, 8, and 12 h. Scale bars, left, 20 µm, right, 5 µm. B The HUVECs migration morphology influenced by PLGA NPs. Scale bar, 100 µm. C The quantification of PLGA NPs uptake by HUVECs (n = 5). D Cell migration area of HUVECs influenced by PLGA NPs (n = 5). E Different concentrations of PLGA NPs and time points impact on the viability of HUVECs. (n = 3) *p < 0.05; **p < 0.01; ***p < 0.001