Fig. 2

Design and characterization of the nanocarriers. A Schematic illustration of the preparation of nanocarriers (top) and schematic of the ErbB2-CAR plasmid (bottom). B Agarose gel retardation assay of the CAR plasmid at various weight ratios. C Zeta potentials and D diameters of nanoparticles containing plasmid DNA with various weight ratios. E Transmission electron microscopy (TEM) image of representative P/PB/N/R nanoparticles. Scale bar, 200 nm. F Zeta potentials and G diameters of nanoparticles containing plasmid DNA with 15:1 weight ratios and P/PB/N/R nanoparticles