Fig. 4

Antitumor efficacy of P/PB/N/R in an orthotopic intracranial BSG model. A Schematic illustration of the experimental design. B IVIS spectrum images and C quantification of the tumor signal of the mice after treatment with different nanoformulations. Each line represents one animal, and each dot reflects its whole animal photon count. D H&E staining of brain tissue sections obtained from mice bearing tumors. E In vivo bioluminescence quantified tumor signal intensity of Fig. 4B at day 21. F Flow cytometry of EGFP-positive cells at different time points after injection of P/PB/N/R nanoparticles. G Changes in body weight during the treatment period. Data are the mean ± s.d. (n = 6). H Survival curve of BSG mice treated with each formulation (n = 6 per group). Statistical significance was calculated via a two-tailed Student’s t test. **P < 0. 01; ***P < 0.001; ****P < 0.0001